Accession Number : ADA332866

Title :   Use of p53 Mutation Analysis for Staging Breast Cancer

Descriptive Note : Annual rept. 1 Aug 96-31 Jul 97

Corporate Author : ONCORMED INC GAITHERSBURG MD

Personal Author(s) : Carter, Christine

PDF Url : ADA332866

Report Date : AUG 1997

Pagination or Media Count : 23

Abstract : Mutations in p53 are found in approximately half of all cancers in man. Their presence, location and molecular characteristics reveal important clues about the etiology of the cancer, the specific DNA transformations that occur, and the spread of disease. The presence of p53 mutations in draining lymph nodes suggests the tumor has already spread systematically. Mutations in the p53 gene have shown to be correlated with outcome and may have direct relevance for staging breast cancer. We used direct sequencing of the p53 gene for mutations in the tumor, and when found, to perform oligonucleotide hybridization of the DNA from the margins and matched lymph nodes draining the primary tumor. Our approach involves the examination of archival specimens from a sample of node-negative breast cancer cases from the American Registry of Pathology, Armed Forces Institute of Pathology. Primary tumor DNA and sequence exons 5-9 of the p53 gene (the region. accounting for 95% of the known mutations) are amplified. For those patients with a mutation, the surgical margins and matching nodal tissue DNA are amplified and tumor-specific DNA probes are created to detect the presence of mutant p53 cells.

Descriptors :   *BREAST CANCER, NEOPLASMS, DEOXYRIBONUCLEIC ACIDS, MUTATIONS, ETIOLOGY, PATHOLOGY, STAGING, BIOASSAY, LYMPH NODES, METASTASIS.

Subject Categories : Genetic Engineering and Molecular Biology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE