Accession Number : ADA333230
Title : Suppressor Genes in Breast Cancer
Descriptive Note : Annual rept 1 Jul 96-30 Jun 97
Corporate Author : GEORGETOWN UNIV WASHINGTON DC
Personal Author(s) : Clarke, Robert ; Pu, Le-Ping
PDF Url : ADA333230
Report Date : JUL 1997
Pagination or Media Count : 20
Abstract : The current proposal will focus on the isolation and characterization of novel TSG(s) in human breast cancer. A human epithelial eukaryotic cDNA expression library has been constructed, and will be transfected into the human breast cancer cell line MCF-7. Gene(s) inhibiting the growth of MCF-7 cells will be considered candidate TSGs for breast cancer. These will be cloned and the full length cDNA sequence obtained. Expression of cloned genes will first be investigated in RNA populations derived from two immortalized "normal" human breast epithelial cell lines (AlN4 and MCF-lOA), and in MCF-7 cells growth arrested either by antiestrogen- treatment or estrogen withdrawal.
Descriptors : *GENES, *SUPPRESSORS, *BREAST CANCER, EPITHELIUM, HUMANS, ISOLATION, NEOPLASMS, DEOXYRIBONUCLEIC ACIDS, MUTATIONS, CLONES, RIBONUCLEIC ACIDS, ESTROGENS.
Subject Categories : Biochemistry
Genetic Engineering and Molecular Biology
Anatomy and Physiology
Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE