Accession Number : ADA333230

Title :   Suppressor Genes in Breast Cancer

Descriptive Note : Annual rept 1 Jul 96-30 Jun 97

Corporate Author : GEORGETOWN UNIV WASHINGTON DC

Personal Author(s) : Clarke, Robert ; Pu, Le-Ping

PDF Url : ADA333230

Report Date : JUL 1997

Pagination or Media Count : 20

Abstract : The current proposal will focus on the isolation and characterization of novel TSG(s) in human breast cancer. A human epithelial eukaryotic cDNA expression library has been constructed, and will be transfected into the human breast cancer cell line MCF-7. Gene(s) inhibiting the growth of MCF-7 cells will be considered candidate TSGs for breast cancer. These will be cloned and the full length cDNA sequence obtained. Expression of cloned genes will first be investigated in RNA populations derived from two immortalized "normal" human breast epithelial cell lines (AlN4 and MCF-lOA), and in MCF-7 cells growth arrested either by antiestrogen- treatment or estrogen withdrawal.

Descriptors :   *GENES, *SUPPRESSORS, *BREAST CANCER, EPITHELIUM, HUMANS, ISOLATION, NEOPLASMS, DEOXYRIBONUCLEIC ACIDS, MUTATIONS, CLONES, RIBONUCLEIC ACIDS, ESTROGENS.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE