Accession Number : ADA333301

Title :   Biophysical Studies of the Type 1 Repeats of Human Thrormbospondin-1 to Characterize the Structural Basis of Its Angiostatic Effect.

Descriptive Note : Annual rept. 1 Aug 96-31 Jul 97

Corporate Author : WISCONSIN UNIV-MADISON

Personal Author(s) : Huwiler, Kristin G. ; Mosher, Deane F.

PDF Url : ADA333301

Report Date : AUG 1997

Pagination or Media Count : 26

Abstract : Thrombospondin-1 (TSP1) is a modular trimeric protein wit several documented functions, including its role as an angiogenic inhibitor. TSP1, TSP1 fragments, and certain TSP1 conserved peptide sequences have been shown to exert an endothelial-specific inhibition of growth and migration. Peptides derived from conserved sequences within the type 1 repeats of TSP1 have been shown to block neo-vascularization in vivo, inhibit DNA synthesis, and migration of cultured endothelial cells in vitro. Our laboratory has shown that recombinantly expressed human TSP1 (hTSP1) type 1 repeats inhibit migration of bFGF stimulated bovine adrenal microvascular endothelial cells. This study seeks to define the structural basis for the angiostatic effect of the hTSP1 type 1 repeats. I will employ biophysical methods in a comparative study of TSP 1 type 1 repeats and active peptides based on type 1 sequences. I have successfully generated recombinant baculoviruses that express the three type 1 repeats in tandem (P123) and the third type 1 repeat (P3) as histidine-tagged fusion proteins. A purification scheme for the recombinant proteins including removal of the histidine-tag has been established. To date, N-terminal sequencing, carbohydrate analysis, and circular dichroism have been performed.

Descriptors :   *PEPTIDES, *IN VITRO ANALYSIS, *BIOSYNTHESIS, *ENDOTHELIUM, PROTEINS, DEOXYRIBONUCLEIC ACIDS, SEQUENCES, PURIFICATION, CELLS(BIOLOGY), CARBOHYDRATES, CIRCULAR, DICHROISM, BIOPHYSICS.

Subject Categories : Biochemistry
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE