Accession Number : ADA334403
Title : Molecular Study of Interactions Between P-Glycoprotein and Anticancer Drugs.
Descriptive Note : Final rept. 1 Aug 94-31 Jul 97
Corporate Author : TEXAS UNIV AT GALVESTON
Personal Author(s) : Zhang, Jian-Ting
PDF Url : ADA334403
Report Date : AUG 1997
Pagination or Media Count : 20
Abstract : P-glycoprotein is a plasma membrane protein that functions as a drug transporter and is responsible for multidrug resistance in some breast cancers. In the past one year, we have generated two site-specific antibodies and used them to determine the topologies of P-glycoprotein in multidrug resistant cancer cells. We found that P-glycoproteins in the plasma membrane of mammalian cells express at least two alternate topologies. This observation is consistent with our previous study using cell-free expression system. The more than one topology feature of P-glycoprotein may be responsible for its multifunctional nature. We have also been able to express the transmembrane domains of P-glycoprotein in bacteria. The success in this study will allow us to map the drug-binding domain in P-glycoprotein and study the drug-P-glycoprotein interactions.
Descriptors : *MEMBRANES(BIOLOGY), *BLOOD PLASMA, *DRUG TOLERANCE, *BREAST CANCER, CARGO VEHICLES, MAMMALS, BACTERIA, PLASMAS(PHYSICS), TOPOLOGY, MOLECULE MOLECULE INTERACTIONS, DRUGS, CELLS(BIOLOGY), ANTIBIOTICS, BLOOD PROTEINS.
Subject Categories : Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE