Accession Number : ADA334885

Title :   Regulation of Growth Factor Release By Proteases Inhibitors in Breast Cancer

Descriptive Note : Annual rept 15 Aug 96-14 Aug 97

Corporate Author : NEW YORK UNIV MEDICAL CENTER NY

Personal Author(s) : Finlay, Thomas H.

PDF Url : ADA334885

Report Date : SEP 1997

Pagination or Media Count : 18

Abstract : This report describes the cloning, sequencing and properties of a potential TGFa-releasing protease present in human breast epithelial cells. Degenerate oligonucleotides based on the conserved sequences about the his(57)and ser(195) residues in mammalian serine proteases were used to clone a novel serine protease sequence (PCR-7) from an MCF-7 breast cancer cell cDNA library. The deduced 321 amino acid open reading frame shows a high degree of homology with the cell-surface serine proteases enterokinase, prekalikrein, hepsin and prostasin. Northern blot analysis, using a PCR-7 cDNA probe, shows the presence of an approximately 4.2 kb mRNA species in total RNA from MCF-7 cells.

Descriptors :   *CLONES, *GROWTH(PHYSIOLOGY), *BREAST CANCER, EPITHELIUM, PROTEINS, DEOXYRIBONUCLEIC ACIDS, GENES, GENETIC ENGINEERING, CELLS(BIOLOGY), RIBONUCLEIC ACIDS, AMINO ACIDS, OLIGOMERS, MAMMARY GLANDS, PEPTIDE HYDROLASES, SERINE, NUCLEOTIDES, ENZYME INHIBITORS.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE