Accession Number : ADA334919
Title : Transgenic Mouse Model to Study the Role of EGF Receptor in Breast Cancer
Descriptive Note : Annual rept 19 Aug 96-18 Aug 97
Corporate Author : ILLINOIS UNIV AT CHICAGO CIRCLE
Personal Author(s) : Chen, William
PDF Url : ADA334919
Report Date : SEP 1997
Pagination or Media Count : 14
Abstract : Epidermal growth factor (EGF) receptor is expressed in many normal tissues, including mammary epithelium. Overexpression or mutation of the EGF receptor cause neoplastic transformation in many cell types. Several studies have implicated the EGF receptor as a good prognostic marker in breast cancer, but its role in the etiology and progression of this malignancy is still under dispute. We propose to utilize a genetic approach to investigate this issue, by developing transgenic mice in which the EGF receptor gene in the mammary gland will be inactivated at the onset of first lactation. We have made the construct for the gene targeting and have 5 individual embryonic stem (ES) cells with potential correct DNA recombination to generate the loxP mouse. We also have made the final construct to generate white acid protein(WAP)-cre transgenic mouse.
Descriptors : *EPITHELIUM, *GROWTH(PHYSIOLOGY), *BREAST CANCER, TISSUES(BIOLOGY), NEOPLASMS, DEOXYRIBONUCLEIC ACIDS, MUTATIONS, ETIOLOGY, TARGETING, MICE, GENETICS, SENSE ORGANS, RECEPTOR SITES(PHYSIOLOGY), TRANSFORMATIONS, EPIDERMIS, MAMMARY GLANDS, LACTATES.
Subject Categories : Biochemistry
Genetic Engineering and Molecular Biology
Anatomy and Physiology
Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE