Accession Number : ADA334919

Title :   Transgenic Mouse Model to Study the Role of EGF Receptor in Breast Cancer

Descriptive Note : Annual rept 19 Aug 96-18 Aug 97

Corporate Author : ILLINOIS UNIV AT CHICAGO CIRCLE

Personal Author(s) : Chen, William

PDF Url : ADA334919

Report Date : SEP 1997

Pagination or Media Count : 14

Abstract : Epidermal growth factor (EGF) receptor is expressed in many normal tissues, including mammary epithelium. Overexpression or mutation of the EGF receptor cause neoplastic transformation in many cell types. Several studies have implicated the EGF receptor as a good prognostic marker in breast cancer, but its role in the etiology and progression of this malignancy is still under dispute. We propose to utilize a genetic approach to investigate this issue, by developing transgenic mice in which the EGF receptor gene in the mammary gland will be inactivated at the onset of first lactation. We have made the construct for the gene targeting and have 5 individual embryonic stem (ES) cells with potential correct DNA recombination to generate the loxP mouse. We also have made the final construct to generate white acid protein(WAP)-cre transgenic mouse.

Descriptors :   *EPITHELIUM, *GROWTH(PHYSIOLOGY), *BREAST CANCER, TISSUES(BIOLOGY), NEOPLASMS, DEOXYRIBONUCLEIC ACIDS, MUTATIONS, ETIOLOGY, TARGETING, MICE, GENETICS, SENSE ORGANS, RECEPTOR SITES(PHYSIOLOGY), TRANSFORMATIONS, EPIDERMIS, MAMMARY GLANDS, LACTATES.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE