Accession Number : ADA335102

Title :   C-KIT AND Stem Cell Factor Expression in Breast Cancer

Descriptive Note : Annual rept 1 Sep 96-31 Aug 97

Corporate Author : VIRGINIA COMMONWEALTH UNIV RICHMOND

Personal Author(s) : Hines, Susan

PDF Url : ADA335102

Report Date : SEP 1997

Pagination or Media Count : 23

Abstract : We have proceeded to investigate some of the biologic consequences of c-kit and SCF coexpression since simple coexpression of receptor and ligand may imply, but does not prove, a potential autocrine(or paracrine) loop. We have therefore, constructed an autocrine loop by transfecting the breast cancer cell line, MCF7, that only expresses SCF, with a c-kit expression vector (21). MCF7 cells were transfected with both the c-kit expression vector and the empty pCEP4 vector (to serve as a control). Since the vector contained a hygromycin resistance gene, stable transfectants could be selected based on their survival in hygromycin-containing media.

Descriptors :   *BREAST CANCER, STIMULATION(PHYSIOLOGY), PROTEINS, NEOPLASMS, GENES, CELL DIVISION, CELLS(BIOLOGY), RIBONUCLEIC ACIDS, KITS, GROWTH(PHYSIOLOGY), HEMATOPOIETIC CELLS, PHOSPHORUS TRANSFERASES, TRANSFECTION.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE