Accession Number : ADA335185

Title :   Detection and Characterization of Autoantigens in Breast Cancer.

Corporate Author : MONTEFIORE HOSPITAL AND MEDICAL CENTER BRONX NY

Personal Author(s) : Racevskis, Janis

PDF Url : ADA335185

Report Date : AUG 1997

Pagination or Media Count : 14

Abstract : The entire sequence of our second breast tumor associated autoantigen (Auag2), which is a newly discovered gene product, has been determined. The predicted amino acid sequence of Auag2 contains two LIM domain motifs, which are conserved cysteine rich zinc-binding motifs of about 60 amino acid residues that mediate protein-protein interactions, and are characteristic of a group of critical transcriptional regulators of embryonic development. The 350 bp region coding for the two tandem LIM domains was found to be 60% homologous to the analogous region of rhombotin 1, a proto-oncogene of 160 amino acids whose gene is disrupted by chromosomal translocation in T-cell leukemia. A third autoantigen clone has been identified and is being characterized. Various features of the Auag2 cDNA sequence (a long GC-rich structured 5' end, the presence of mRNA destabilizing motifs in the 3' end and a homology to a known oncogene) all predict that this gene plays a vital role in the life of the organism, and may play a role in malignancy.

Descriptors :   *NEOPLASMS, *ANTIGENS, *AMINO ACIDS, *MAMMARY GLANDS, *BREAST CANCER, DETECTION, INTERACTIONS, PROTEINS, CHROMOSOMES, REGIONS, SEQUENCES, CODING, GENES, T LYMPHOCYTES, EMBRYOS, LEUKEMIA, TRANSLOCATION.

Subject Categories : Biochemistry
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE