Accession Number : ADA335807

Title :   Molecular Mechanisms of Biosynthesis of Metal-Binding PCs

Descriptive Note : Final rept. 01 Nov 93-31 Oct 97

Corporate Author : CALIFORNIA UNIV RIVERSIDE

Personal Author(s) : Mehra, Rajesh

PDF Url : ADA335807

Report Date : 31 OCT 1997

Pagination or Media Count : 19

Abstract : Our research during the project period was directed towards three fundamental aspects of PCs: (1) metal-chelation by PCs, (2) sulfide complexes of PCs and (3) biosynthesis of PCs. We have made thorough investigations of the metal-binding properties of PCs and published data that provides benchmark information on the Ag(1), Cu(1), Cd(2), Hg(2) and Pb(2)-binding characteristics of these plant metal binding peptides. We have carried out detailed investigations on the sulfide complexes of PCs (PCs) that profoundly influence the metal-binding capacity of these peptides. In addition, these complexes have the potential to be very useful reagents for the detoxification of a variety of organic contaminants. Using a complementation cloning strategy, we have isolated a gene that is required for Cd tolerance and PC production. Although this gene did not turn out to be PC synthase, it provides us a good picture of the role of heme biosynthesis in PC production.

Descriptors :   *PEPTIDES, *MOLECULAR PROPERTIES, *BIOSYNTHESIS, METALS, COMPLEX COMPOUNDS, CHELATION, COPPER, ORGANIC MATERIALS, MERCURY, CONTAMINANTS, CADMIUM, GENES, SYNTHASES, BINDERS, HEMOGLOBIN, SILVER, SULFIDES, DETOXIFICATION, LEAD(METAL).

Subject Categories : Biochemistry
      Inorganic Chemistry
      Organic Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE