Accession Number : ADA335915

Title :   Wnt-5a and Wnt-4 Regulates Cell Growth in CS7MG Mammary Epithelial Cells

Descriptive Note : Annual rept 1 Jul 96-30 Jun 97

Corporate Author : MILTON S HERSHEY MEDICAL CENTER PA

Personal Author(s) : Olson, Daniel J.

PDF Url : ADA335915

Report Date : JUL 1997

Pagination or Media Count : 34

Abstract : The implications for determining whether wnt-5a and wnt-4 act as mediators of normal cell growth are relevant to the detection, diagnosis, and treatment of breast cancer. The intent of this proposal has been to develop new mammary epithelial cell lines which stably express antisense wnt-5a or wnt-4 in order to determine whether the loss of endogenous gene expression of either is important for cell transformation to occur. It was found that disruption of wnt-5a leads to cell transformation and ectopic wnt-5a gene expression in transformed cells reverts cells to a more differentiated phenotype.

Descriptors :   *EPITHELIUM, *GENES, *BREAST CANCER, DETECTION, ENZYMES, CHROMOSOMES, CELLS(BIOLOGY), TRANSFORMATIONS, GENETIC MAPPING, GROWTH(PHYSIOLOGY).

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE