Accession Number : ADA335925

Title :   A Novel 8OkDa Matrix-Degrading Proteinase in Breast Cancer Invasion and Metastasis

Descriptive Note : Annual rept 1 Sep 96-31 Aug 97

Corporate Author : LONG ISLAND JEWISH-HILLSIDE MEDICAL CENTER NEW HYDE PARK NY

Personal Author(s) : Shi, Yuenian E.

PDF Url : ADA335925

Report Date : SEP 1997

Pagination or Media Count : 42

Abstract : A novel breast cancer specific gene BCSG1 was cloned by differential cDNA sequencing. BCSG1, a Alzheimer's disease (AD) amyloid like protein, is expressed in high abundance in breast cancer cDNA library but scarcely in normal breast cDNA library. Preliminary studies suggest that: (1) BCSG1 expression is a stage-specific in human breast: undetectable in normal or benign breast lesions, low level and partial expression in low grade ductal carcinoma in situ but extremely high level in advanced infiltrating breast cancer; (2) overexpression of BCSG1 in human breast cancer cells stimulates cell growth and invasion potentials in vitro and tumor growth in nude mice; (3) BCSG1 expression in breast cancer cells is dramatically suppressed by tumor growth inhibitor oncostatin M (OM), a cytokine predominantly produced by activated T cells and macrophages. This OM-induced transcriptional suppression of BCSG1 gene is associated with OM-mediated growth inhibition. The overall hypothesis to be evaluated is that overexpression of BCSG1 may correlate with clinical aggressiveness of breast cancer and lead to their abnormal growth and malignant progression. We will study the biological functions of BCSG1 on breast cancer growth and metastasis; to define its range of expression and begin to determine the mechanisms whereby BCSG1 expression is involved in breast cancer progression.

Descriptors :   *ENZYMES, *METASTASIS, *BREAST CANCER, STIMULATION(PHYSIOLOGY), HUMANS, PROTEINS, NEOPLASMS, DEOXYRIBONUCLEIC ACIDS, IN VITRO ANALYSIS, GENES, CLONES, MICE, INHIBITORS, CELLS(BIOLOGY), MACROPHAGES, MAMMARY GLANDS, GROWTH(PHYSIOLOGY), PEPTIDE HYDROLASES.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE