Accession Number : ADA335944

Title :   A Genetic Screen for Ligand Binding by the Human Estrogen Receptor

Descriptive Note : Annual rept 15 Aug 96-14 Aug 97

Corporate Author : EUROPEAN MOLECULAR BIOLOGY LAB HEIDELBERG (GERMANY)

Personal Author(s) : Nichols, Mark D.

PDF Url : ADA335944

Report Date : SEP 1997

Pagination or Media Count : 49

Abstract : FLP recombinase-steroid receptor fusion proteins convert ligand binding into DNA recombination in yeast and mammalian cells. We describe a ligand responsive FLP-estrogen receptor binding domain (FLP-EBD) in yeast that reflects known estrogen affinities. Shortening the distance between FLP and the EBD in the fusion protein specifically blocks induction only by antagonists, presenting an assay for them in yeast. We show, using this assay for ligand binding that does not rely on transcriptional responsiveness, that agonists and antagonists differently position the C-terminus of the ligand binding domain (helix 12) and the F domain.

Descriptors :   *LIGANDS, *ESTROGENS, *BREAST CANCER, HORMONES, HUMANS, ENZYMES, PROTEINS, DEOXYRIBONUCLEIC ACIDS, MUTATIONS, IN VITRO ANALYSIS, YEASTS, INVERSION, CELLS(BIOLOGY), GENETICS, RECEPTOR SITES(PHYSIOLOGY), STEROIDS.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE