Accession Number : ADA336394

Title :   Pgp Concentration of Carcinogens in Breast Epithelia.

Descriptive Note : Annual rept 1 Sep 96-31 Aug 97

Corporate Author : MEDICAL UNIV OF SOUTH CAROLINA CHARLESTON

Personal Author(s) : Karnaky, Karl J.

PDF Url : ADA336394

Report Date : SEP 1997

Pagination or Media Count : 11

Abstract : We are examining the possibility that the membrane transporter, Pgp, could continually or episodically (under the influence of various steroid hormones or environmental factors) transport hydrophobic toxic molecules, including pesticides, into the lumen of breast epithelia over long periods of time (years). Toxins transported into the lumen would be concentrated well above blood concentrations and would continue to diffuse from the lumen back to the cell, affording them time to damage or modify DNA. Published immunocytochemical studies reveal that Pgp is resent in human breast ductules, but we have not seen any functional evidence for its presence. We have preliminary evidence for another transports, the multispecific organic anion transporter (MOAT), which transports fluorescent methotrexate into ductule lumens. A second phase of this research, utilizing primary tissue cultures and cell lines of human breast epithelial tissue. We will use these model systems to determine transporter substrates and potential hormonal regulation of these transporters.

Descriptors :   *EPITHELIUM, *CARCINOGENS, *BREAST CANCER, HORMONES, TISSUES(BIOLOGY), FLUORESCENCE, TOXICITY, SUBSTRATES, DEOXYRIBONUCLEIC ACIDS, MEMBRANES, CELLS(BIOLOGY), ANIONS, TOXINS AND ANTITOXINS, PESTICIDES, MAMMARY GLANDS, IMMUNOCHEMISTRY, STEROIDS, METHOTREXATE.

Subject Categories : Biochemistry
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE