Accession Number : ADA336520

Title :   The Effect of Radiotherapy Upon Primary Human Mammary Epithelial Cells which Harbor a Breast Cancer Susceptibility Gene.

Descriptive Note : Annual rept. 1 Sep 96-31 Aug 97,

Corporate Author : MICHIGAN UNIV ANN ARBOR

Personal Author(s) : Pierce, Lori

PDF Url : ADA336520

Report Date : SEP 1997

Pagination or Media Count : 17

Abstract : The purpose of this work is to determine whether breast cancer predisposition is correlated with enhanced cellular sensitivity to radiotherapy. Therefore mammary epithelial cells from women at high risk for carrying either a BRCA-1 or BRCA-2 germline mutation will be compared with mammary cells from women not at risk for carrying a mutation following radiation using the following assays: growth factor independence, growth in soft agar, and tumorigenicity in vivo. The work done thus far has largely focused on characterizing the high risk cell lines. All baseline radiation experiments have been done and the growth factor experiments have begun, which show a dependence upon EGF in unirradiated high risk (and control) cell lines.

Descriptors :   *GENES, *MEDICAL RESEARCH, *CELLS(BIOLOGY), *MAMMARY GLANDS, *RADIOTHERAPY, *BREAST CANCER, EPITHELIUM, HIGH RATE, RISK, GROWTH(GENERAL), SENSITIVITY, DEOXYRIBONUCLEIC ACIDS, MUTATIONS, BASE LINES, WOMEN, RADIATION DAMAGE, AGAR.

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research
      Radiobiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE