Accession Number : ADA337015

Title :   Oligosaccharide Markers for Prognosis of Low-Risk Breast Cancer.

Descriptive Note : Annual rept. 1 Sep 96-31 Aug 97.

Corporate Author : COLORADO UNIV HEALTH SCIENCES CENTER DENVER

Personal Author(s) : Pettijohn, David E.

PDF Url : ADA337015

Report Date : SEP 1997

Pagination or Media Count : 8

Abstract : Progress to date has included the determination of the occurrence of several different cell surface oligosaccharides and proteases on sections from 181 different ductal breast carcinomas. Monoclonal antibodies specific for the blood group related oligosaccharides Lea, LeX, sialyl- Lea, LeY, Tn, Sialyl Tn and extended Lea - LeX were used to define the cell surface oligosaccharides and both immunofluorescence techniques and immuno streptavadin-biotin techniques were used to evaluate the fraction of tumor cells that are positive and the relative amount of reaction product as measured using an image processing system. The preliminary analysis of the available data has shown that the extended Lea-LeX oligosaccharide is a statistically significant indicator of poor prognosis following surgical removal of small, node-negative ductal breast carcinomas. cDNA libraries constructed from cells that express Lea-LeX have been screened and selected for cDNAs that alter the expression of this oligosaccharide in cancer cells. Three candidate genes have been identified and preliminarily characterized.

Descriptors :   *NEOPLASMS, *SACCHARIDASES, *MONOCLONAL ANTIBODIES, *MAMMARY GLANDS, *BREAST CANCER, IMAGE PROCESSING, RISK, PREDICTIONS, CELLS, GENES, CELLS(BIOLOGY), CARBOHYDRATES, DETERMINATION, CANCER, PEPTIDE HYDROLASES, BLOOD GROUPS.

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE