Accession Number : ADA337017

Title :   Mammary Tumor Development: Stromal-Epithelial Interactions in Oncogenesis.

Descriptive Note : Annual rept. 1 Sep 96-31 Aug 97.

Corporate Author : THOMAS JEFFERSON UNIV PHILADELPHIA PA

Personal Author(s) : Strayer, Davud S.

PDF Url : ADA337017

Report Date : SEP 1997

Pagination or Media Count : 25

Abstract : The purpose of this grant is to define interactions between mammary stroma and epithelium in oncogenesis in transgenic mice expressing Shope growth factor (SGF). Different lines of SGF transgenic mice express this EGF-like cytokine using inducible (metallothionein, MT) or constitutive (RSV-LTR) promoters. We have mostly fulfilled, and in some areas exceeded, proposed goals for the year now ending. We had proposed making sufficient SGF-transgenic mice of RSV-SGF and MT-SGF lines for analysis of mammary differentiation and tumor development. This included assessing expression of SGF and of key cellular genes important in mammary these processes. It also included defining which cells express the transgene and these cellular genes. These studies are completed. We have also interbred RSV- and MT-SGF transgenic mice with p53-/- mice, to assess the effects of a homozygous deletion of this tumor suppressor gene on mammary gland development and tumorigenesis. These mice are just numerous enough for these analyses to be performed. We have been producing re-engineered SGF in baculovirus in quantities that can be used for study of SGF activity in cultured cells as proposed. Therefore, our studies of the effects of SGF on mammary gland and tumor development are proceeding as planned, and additional studies are in progress.

Descriptors :   *EPITHELIUM, *NEOPLASMS, *MAMMARY GLANDS, *GROWTH(PHYSIOLOGY), *ONCOGENIC VIRUSES, *BREAST CANCER, PRODUCTION, CELLS, GENES, MICE, SUPPRESSORS.

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE