Accession Number : ADA337537

Title :   The Role of p53 in Human Breast Cancer

Descriptive Note : Final rept 1 Sep 94-31 Aug 97

Corporate Author : TEXAS UNIV SOUTHWESTERN MEDICAL SCHOOL AT DALLAS

Personal Author(s) : Shay, Jerry W.

PDF Url : ADA337537

Report Date : SEP 1997

Pagination or Media Count : 80

Abstract : The technical objectives of this project were to: (1) transfect mutant P53s commonly mutated in human breast cancer into normal human mammary epithelial cells obtained from different donors and isolate clones; (2) characterize the clones for extension of lifespan and immortalization; (3) determine if expression of any of the mutant p53s provide a growth advantage to breast epithelial cells prior to immortalization; (4) determine in breast epithelial cells immortalized and expressing mutant p53 if expression of the mutant p53 is necessary for the maintenance of growth; and (5) determine downstream genomic targets of p53 that may be important in the development and progression of breast cancer. The technical objectives accomplished include the determination of the effectiveness of different p53 mutants in extension of breast epithelial lifespan and immortalization; the spontaneous immortalization of Li-Fraumeni breast epithelial cells; the determination of the effectiveness of different promoters for achieving extension of lifespan and immortalization; and the determination of the role of telomerase in the development of human breast cancer.

Descriptors :   *PROTEINS, *BREAST CANCER, EPITHELIUM, HUMANS, ENZYMES, MUTATIONS, CLONES, LIFE SPAN(BIOLOGY), CELLS(BIOLOGY), MAMMARY GLANDS, SUPPRESSORS, GROWTH(PHYSIOLOGY).

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE