Accession Number : ADA337537
Title : The Role of p53 in Human Breast Cancer
Descriptive Note : Final rept 1 Sep 94-31 Aug 97
Corporate Author : TEXAS UNIV SOUTHWESTERN MEDICAL SCHOOL AT DALLAS
Personal Author(s) : Shay, Jerry W.
PDF Url : ADA337537
Report Date : SEP 1997
Pagination or Media Count : 80
Abstract : The technical objectives of this project were to: (1) transfect mutant P53s commonly mutated in human breast cancer into normal human mammary epithelial cells obtained from different donors and isolate clones; (2) characterize the clones for extension of lifespan and immortalization; (3) determine if expression of any of the mutant p53s provide a growth advantage to breast epithelial cells prior to immortalization; (4) determine in breast epithelial cells immortalized and expressing mutant p53 if expression of the mutant p53 is necessary for the maintenance of growth; and (5) determine downstream genomic targets of p53 that may be important in the development and progression of breast cancer. The technical objectives accomplished include the determination of the effectiveness of different p53 mutants in extension of breast epithelial lifespan and immortalization; the spontaneous immortalization of Li-Fraumeni breast epithelial cells; the determination of the effectiveness of different promoters for achieving extension of lifespan and immortalization; and the determination of the role of telomerase in the development of human breast cancer.
Descriptors : *PROTEINS, *BREAST CANCER, EPITHELIUM, HUMANS, ENZYMES, MUTATIONS, CLONES, LIFE SPAN(BIOLOGY), CELLS(BIOLOGY), MAMMARY GLANDS, SUPPRESSORS, GROWTH(PHYSIOLOGY).
Subject Categories : Biochemistry
Genetic Engineering and Molecular Biology
Anatomy and Physiology
Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE