Accession Number : ADA337551

Title :   Human Adrenal Androgens: Regulation of Biosynthesis and Role in Estrogen-Responsive Breast Cancer in a Mouse Model.

Descriptive Note : Annual rept. 1 Sep 96-31 Aug 97

Corporate Author : BAYLOR COLL OF MEDICINE HOUSTON TX

Personal Author(s) : Hornsby, Peter J.

PDF Url : ADA337551

Report Date : SEP 1997

Pagination or Media Count : 7

Abstract : These experiments investigate a mouse model of human adrenal androgen biosynthesis and the role of these steroids in human breast cancer growth. An androgen-dependent human breast cancer model was established in the scid mouse. To provide zona reticularis function, essential for adrenal androgen biosynthesis, in human adrenal organoids in the mouse, two approaches are being taken; first, to form an organoid with a capillary bed adequate for proper zonation to be re established, and second, the genetic engineering of clonal adrenal cells to suppress 3 13-hydroxysteroid dehydrogenase (3%-HSD), the key enzyme regulating DMEA biosynthesis. Clonal bovine adrenal cells tormed a fimctional organoid in scid mice that replaced the animals' adrenal function. Southwestern blotting using a probe in the regulatory region of the 3p-HSD gene has shown zonal differences in protein binding. The characterization of these transcription factors may provide future information on the molecular basis of zonation and thus indicate methods for obtaining zona reticularis function in the organoids.

Descriptors :   *CELLS(BIOLOGY), *BIOSYNTHESIS, *ADRENAL GLANDS, *ESTROGENS, *BREAST CANCER, CONTROL, HUMANS, ENZYMES, PROTEINS, ANIMALS, RESPONSE(BIOLOGY), MICE, GENETIC ENGINEERING, BOVINES, ANATOMICAL MODELS, GROWTH(PHYSIOLOGY), STEROIDS, ANDROGENS.

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE