Accession Number : ADA337846

Title :   Neuropharmacological Characterization of Botulinum Neurotoxin.

Descriptive Note : Final rept. 1 Apr 95-31 Mar 97,

Corporate Author : THOMAS JEFFERSON UNIV PHILADELPHIA PA

Personal Author(s) : Simpson, Lance L.

PDF Url : ADA337846

Report Date : APR 1997

Pagination or Media Count : 42

Abstract : The binding of iodinated botulinum toxin to nerve membranes was studied by using rat and mouse preparations. The toxin was examined both in the single-chain and in the proteolytically processed dichain form, and binding sites both in the spinal cord and in various brain regions were assayed. Rat and mouse brains possessed specific binding sites for botulinum toxin. The binding of botulinum toxin to rat brain membranes was not antagonized by a polyclonal antibody against the cytosolic domain of synaptotagmin 1 or by a monoclonal antibody directed against the luminal domain of synaptotagmin 1. In addition, these antibodies did not protect the mouse phrenic nerve-hemidiaphragm from toxin-induced neuromuscular blockade. Extraction of whole-brain niRNA and injection into Xenopus oocytes led to expression of binding sites for botulinum toxin. Extraction and injection of cerebellar mRNA led to expression of a higher density of binding sites. The number of binding sites was not diminished when oocytes were pretreated with antibodies against the cytosolic and luminal domains of synaptotagmin 1.

Descriptors :   *ANTIBODIES, *CLOSTRIDIUM BOTULINUM, *NEUROTOXINS, DENSITY, BRAIN, RATS, MEMBRANES, MICE, MONOCLONAL ANTIBODIES, NERVES, BACTERIAL TOXINS, SPINAL CORD.

Subject Categories : Biochemistry
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE