Accession Number : ADA338374

Title :   Guidance for Performance of the H4IIE Dioxin Screening Assay.

Corporate Author : ARMY ENGINEER WATERWAYS EXPERIMENT STATION VICKSBURG MS

PDF Url : ADA338374

Report Date : FEB 1998

Pagination or Media Count : 24

Abstract : This technical note provides protocols for maintenance of cell cultures and for the conduct of a biomarker-based screening assay for dioxin toxic equivalents (TCDD TEQs) in sediments and other environmental samples using the H4IIE rat hepatoma cell line as performed at the U.S. Army Engineer Waterways Experiment Station (WES). The purpose of these protocols is to provide analysts who may wish to gain the capability for performing the assay with a detailed 'blueprint' of how the assay is conducted and of what is necessary in terms of technique, materials, instrumentation, and time.

Descriptors :   *CELLS(BIOLOGY), *CULTURES(BIOLOGY), *TOXIC DISEASES, *DIOXINS, MAINTENANCE, ENVIRONMENTS, SAMPLING, SEDIMENTS.

Subject Categories : Medicine and Medical Research
      Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE