Accession Number : ADA338716
Title : A Quantitative Multimarker Molecular Assay to Detect Hemtogenous Breast Cancer Metastasis
Descriptive Note : Annual rept. 1 Jul 96-30 Jun 97
Corporate Author : JOHN WAYNE INST FOR CANCER TREATMENT AND RESEARCH SANTA MONICA CA
Personal Author(s) : Hoon, Dave S.
PDF Url : ADA338716
Report Date : AUG 1997
Pagination or Media Count : 24
Abstract : Clinical detection of tumor progression is a major problem in treatment decisions for breast cancer patients. The ability to accurately detect hematogenous metastasis should significantly aid oncologist in disease management. To identify tumor progression more effectively than current approaches a less invasive, more accurate, quantitative assay is needed. Detection of metastatic tumor cells in blood and tissue by single marker Reverse- Transcriptase Polymerase Chain Reaction (RT-PCR) has shown potential value in clinical diagnosis. We have successfully developed unique approach using multimarker, quantitative analysis and automated Southern blot detection system to provide highly stringent large scale analysis. Our experience in other cancers and viral nucleic detection will be applied to breast cancer. In this proposal we plan to test our hypothesis that a multimarker molecular assay can be clinically useful in detection hematogenous metastasis in breast cancer patients of different clinical and pathological stages of disease. The objective is to improve diagnosis and monitor patients for metastatic disease.
Descriptors : *METASTASIS, *BREAST CANCER, TISSUES(BIOLOGY), QUANTITATIVE ANALYSIS, DETECTION, MOLECULES, ENZYMES, CLINICAL MEDICINE, NEOPLASMS, DIAGNOSIS(MEDICINE), MARKERS, PATHOLOGY, CELLS(BIOLOGY), ASSAYING, RIBONUCLEIC ACIDS, CHAIN REACTIONS, BLOOD.
Subject Categories : Biochemistry
Genetic Engineering and Molecular Biology
Anatomy and Physiology
Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE