Accession Number : ADA338716

Title :   A Quantitative Multimarker Molecular Assay to Detect Hemtogenous Breast Cancer Metastasis

Descriptive Note : Annual rept. 1 Jul 96-30 Jun 97

Corporate Author : JOHN WAYNE INST FOR CANCER TREATMENT AND RESEARCH SANTA MONICA CA

Personal Author(s) : Hoon, Dave S.

PDF Url : ADA338716

Report Date : AUG 1997

Pagination or Media Count : 24

Abstract : Clinical detection of tumor progression is a major problem in treatment decisions for breast cancer patients. The ability to accurately detect hematogenous metastasis should significantly aid oncologist in disease management. To identify tumor progression more effectively than current approaches a less invasive, more accurate, quantitative assay is needed. Detection of metastatic tumor cells in blood and tissue by single marker Reverse- Transcriptase Polymerase Chain Reaction (RT-PCR) has shown potential value in clinical diagnosis. We have successfully developed unique approach using multimarker, quantitative analysis and automated Southern blot detection system to provide highly stringent large scale analysis. Our experience in other cancers and viral nucleic detection will be applied to breast cancer. In this proposal we plan to test our hypothesis that a multimarker molecular assay can be clinically useful in detection hematogenous metastasis in breast cancer patients of different clinical and pathological stages of disease. The objective is to improve diagnosis and monitor patients for metastatic disease.

Descriptors :   *METASTASIS, *BREAST CANCER, TISSUES(BIOLOGY), QUANTITATIVE ANALYSIS, DETECTION, MOLECULES, ENZYMES, CLINICAL MEDICINE, NEOPLASMS, DIAGNOSIS(MEDICINE), MARKERS, PATHOLOGY, CELLS(BIOLOGY), ASSAYING, RIBONUCLEIC ACIDS, CHAIN REACTIONS, BLOOD.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE