Accession Number : ADA338722

Title :   Actions and Substrates for the HER4 Tyrosine Kinase in Breast Cancer

Descriptive Note : Annual rept. 1 Jul 96-30 Jun 97

Corporate Author : NORTH CAROLINA UNIV AT CHAPEL HILL

Personal Author(s) : Earp, H. S.

PDF Url : ADA338722

Report Date : JUL 1997

Pagination or Media Count : 14

Abstract : During the first year of our HER4 and breast cancer grant, we have made progress in methods, cell line and reagent development. We have made MDA-MB 453 and 32D cell lines that stably express the Epidermal Growth Factor (EGF) receptor:Human EGF receptor 4 (HER4) chimera. Antisera directed against the chimera have demonstrated its expression. These 2 transfected cell lines will be used to compare HER4 and EGF receptor tyrosine phosphorylated substrates. Activation of the EGF receptor:HER4 chimera by adding EGF to transfected 32D cells stops their growth. This result suggest that our overarching hypothesis, i.e., the HER4 signal is different from that of the EGF receptor, is correct. To isolate downstream HER4 signaling elements, we are creating recombinant molecules as bait for use in the yeast 2 hybrid system. We are testing the ability of these constructs to be tyrosine phosphorylated when expressed in yeast. Lastly, generation of a HER4 antibody should allow us to do Western blot analysis of HER4 content in a variety of breast cancer samples, allowing attempts to correlate HER4 expression and breast cancer prognosis.

Descriptors :   *SUBSTRATES, *TYROSINE, *PHOSPHORUS TRANSFERASES, *BREAST CANCER, MOLECULES, DEOXYRIBONUCLEIC ACIDS, ANTIBODIES, YEASTS, GENES, CELLS(BIOLOGY), RECEPTOR SITES(PHYSIOLOGY), EPIDERMIS, GROWTH(PHYSIOLOGY), PHOSPHORYLATION.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE