Accession Number : ADA338766

Title :   Antibody-IL2 Fusion Protein Delivery by Gene Transfer.

Descriptive Note : Annual rept. 1 Oct 96-30 Sep 97,

Corporate Author : WISCONSIN UNIV-MADISON

Personal Author(s) : Nicolet, Charles M.

PDF Url : ADA338766

Report Date : OCT 1997

Pagination or Media Count : 17

Abstract : The purpose of the work described is to assess the feasibility of a gene therapy approach to deliver a specific antibody cytokine fusion protein called CC49-1L2 to a tumor expressing antigen reactive with the antibody. While gene therapy approaches to the delivery of immune stimulating compounds such as cytokines have been widely explored, less has been done investigating other molecules in these contexts. We further were interested in assessing a specific type of gene therapy for this purpose called particle mediated gene transfer in which the DNA of interest is deposited directly into cells using gas propelled gold beads as a delivery vehicle. In our studies, we first showed that a commonly used retroviral derived vector was the most effective at driving expression of the fusion protein. We also attempted to establish an immunotherapy model in which antibody reactive tumors were en grafted into syngeneic immunocompetent murine hosts. Growth of these tumors was monitored following PMGT of the FP at the tumor site. Our initial attempts at exploiting this model were unable to document a statistically significant anti-tumor effect.

Descriptors :   *NEOPLASMS, *ANTIBODIES, *MONOCLONAL ANTIBODIES, *GENETIC ENGINEERING, *BREAST CANCER, DELIVERY, MOLECULES, REACTIVITIES, DEOXYRIBONUCLEIC ACIDS, IN VITRO ANALYSIS, GASES, PROPULSION SYSTEMS, PARTICLES, GENES, TRANSFER, GOLD, IN VIVO ANALYSIS, IMMUNIZATION, EPIDERMIS, BEADS.

Subject Categories : Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE