Accession Number : ADA338776

Title :   Role of Seprase in Breast Cancer

Descriptive Note : Annual rept. 1 Sep 96-31 Aug 97

Corporate Author : ARKANSAS UNIV AT LITTLE ROCK

Personal Author(s) : Kelly, Thomas J., Jr.

PDF Url : ADA338776

Report Date : SEP 1997

Pagination or Media Count : 57

Abstract : This research investigates the role of the serine integral membrane protease seprase in human breast cancer cell invasion. The project goals are to identify and characterize a full-length cDNA fur human breast cancer seprase and to investigate the contribution of seprase to the metastatic potential of breast cells. Progress made in the first year of this four year project is reported. Chicken embryo cDNA libraries have been prepared and screened with the polyclonal antibody against chicken embryo seprase that intensely labels human breast cancer cells. This probe identified a novel gene encoding a protein of unknown function. A reverse transcriptase- polymerase chain reaction (RT-PCR) strategy for cloning the seprase gene was developed based on conserved DNA sequences of human nonclassical serine proteases. A 250 bp cDNA with 85 % homology to catalytic domain of the family of serine integral membrane proteases that includes seprase was produced by RT-PCR of chicken embryo RNA.

Descriptors :   *ENZYMES, *BREAST CANCER, MEMBRANES(BIOLOGY), HUMANS, PROTEINS, DEOXYRIBONUCLEIC ACIDS, CODING, GENES, CLONES, MONOCLONAL ANTIBODIES, GENETIC ENGINEERING, RIBONUCLEIC ACIDS, AMINO ACIDS, MAMMARY GLANDS, PEPTIDE HYDROLASES, SERINE, METASTASIS.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE