Accession Number : ADA338816

Title :   Fluid Transport Mechanisms in Breast Gross Cystic Disease

Descriptive Note : Annual rept. 1 Sep 96-31 Aug 97

Corporate Author : CALIFORNIA UNIV BERKELEY

Personal Author(s) : Miller, Sheldon

PDF Url : ADA338816

Report Date : SEP 1997

Pagination or Media Count : 23

Abstract : The goal of this research is to develop a pharmacological strategy for reducing or eliminating the pathological accumulation of fluid that occurs in breast gross cystic disease (GCD). We have begun an analysis of the transport proteins and intracellular signaling mechanisms that mediate the movement of fluid (and ions) across confluent monolayers of human cultured mammary epithelial cells beginning with a well-characterized breast cancer mammary cell line (31EG4). The electrophysiology data obtained using 31EG4 cultures provides evidence in human mammary cells for apical membrane Cl channels (cAMP and Ca2+-activated) and amiloride sensitive Na channels. The present preliminary experiments have provided the first measurements of fluid transport across any mammary epithelia.

Descriptors :   *DISEASES, *CYSTS, *TISSUE FLUIDS, *BREAST CANCER, EPITHELIUM, HUMANS, PROTEINS, SIGNALS, TRANSPORT, THERAPY, GENES, PATHOLOGY, ABSORPTION, ACCUMULATION, CELLS(BIOLOGY), CULTURES(BIOLOGY), PHARMACOLOGY, MAMMARY GLANDS, ELECTROPHYSIOLOGY.

Subject Categories : Biochemistry
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE