Accession Number : ADA338816
Title : Fluid Transport Mechanisms in Breast Gross Cystic Disease
Descriptive Note : Annual rept. 1 Sep 96-31 Aug 97
Corporate Author : CALIFORNIA UNIV BERKELEY
Personal Author(s) : Miller, Sheldon
PDF Url : ADA338816
Report Date : SEP 1997
Pagination or Media Count : 23
Abstract : The goal of this research is to develop a pharmacological strategy for reducing or eliminating the pathological accumulation of fluid that occurs in breast gross cystic disease (GCD). We have begun an analysis of the transport proteins and intracellular signaling mechanisms that mediate the movement of fluid (and ions) across confluent monolayers of human cultured mammary epithelial cells beginning with a well-characterized breast cancer mammary cell line (31EG4). The electrophysiology data obtained using 31EG4 cultures provides evidence in human mammary cells for apical membrane Cl channels (cAMP and Ca2+-activated) and amiloride sensitive Na channels. The present preliminary experiments have provided the first measurements of fluid transport across any mammary epithelia.
Descriptors : *DISEASES, *CYSTS, *TISSUE FLUIDS, *BREAST CANCER, EPITHELIUM, HUMANS, PROTEINS, SIGNALS, TRANSPORT, THERAPY, GENES, PATHOLOGY, ABSORPTION, ACCUMULATION, CELLS(BIOLOGY), CULTURES(BIOLOGY), PHARMACOLOGY, MAMMARY GLANDS, ELECTROPHYSIOLOGY.
Subject Categories : Biochemistry
Anatomy and Physiology
Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE