Accession Number : ADA338865

Title :   Inhibition of Breast Cancer Growth by IGFBP-3

Descriptive Note : Annual rept. 1 Oct 96-30 Sep 97

Corporate Author : OREGON HEALTH SCIENCES UNIV PORTLAND

Personal Author(s) : Oh, Youngman

PDF Url : ADA338865

Report Date : OCT 1997

Pagination or Media Count : 27

Abstract : The proposal of my grant is to investigate the biological significance and mechanism of IGFBP-3 as well as identification and characterization of the IGFBP-3 receptor in human breast cancer cells. As a first year task, I initiated the identification of the putative IGFBP-3 receptor in human breast cancer cells by employing the yeast two-hybrid system. To date this approach has been successful using total Hs578T breast cancer cell mRNA for generation of a cDNA library, and a cDNA encoding an IGFBP-3 fragment IGFBP-3 sup 88-148 as a "bait". Preliminary screening of transformant colonies was successful, yielding several hundred positive interaction clones.

Descriptors :   *PROTEINS, *INHIBITION, *GROWTH(PHYSIOLOGY), *BREAST CANCER, HUMANS, INTERACTIONS, PEPTIDES, DEOXYRIBONUCLEIC ACIDS, CODING, LIGANDS, HYBRID SYSTEMS, ANTIBODIES, CLONES, INHIBITORS, CELLS(BIOLOGY), RIBONUCLEIC ACIDS, RECEPTOR SITES(PHYSIOLOGY), INSULIN.

Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE