Accession Number : ADA338865
Title : Inhibition of Breast Cancer Growth by IGFBP-3
Descriptive Note : Annual rept. 1 Oct 96-30 Sep 97
Corporate Author : OREGON HEALTH SCIENCES UNIV PORTLAND
Personal Author(s) : Oh, Youngman
PDF Url : ADA338865
Report Date : OCT 1997
Pagination or Media Count : 27
Abstract : The proposal of my grant is to investigate the biological significance and mechanism of IGFBP-3 as well as identification and characterization of the IGFBP-3 receptor in human breast cancer cells. As a first year task, I initiated the identification of the putative IGFBP-3 receptor in human breast cancer cells by employing the yeast two-hybrid system. To date this approach has been successful using total Hs578T breast cancer cell mRNA for generation of a cDNA library, and a cDNA encoding an IGFBP-3 fragment IGFBP-3 sup 88-148 as a "bait". Preliminary screening of transformant colonies was successful, yielding several hundred positive interaction clones.
Descriptors : *PROTEINS, *INHIBITION, *GROWTH(PHYSIOLOGY), *BREAST CANCER, HUMANS, INTERACTIONS, PEPTIDES, DEOXYRIBONUCLEIC ACIDS, CODING, LIGANDS, HYBRID SYSTEMS, ANTIBODIES, CLONES, INHIBITORS, CELLS(BIOLOGY), RIBONUCLEIC ACIDS, RECEPTOR SITES(PHYSIOLOGY), INSULIN.
Subject Categories : Biochemistry
Genetic Engineering and Molecular Biology
Anatomy and Physiology
Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE