Accession Number : ADA396506

Title :   Direct Effects of Folate Metabolism on Gene Expression in Metastatic Breast Cancer

Descriptive Note : Annual rept. 1 Jul 2000-30 Jun 2001

Corporate Author : CORNELL UNIV ITHACA NY

Personal Author(s) : Calero, Monica ; Collins, Ruth N.

PDF Url : ADA396506

Report Date : JUL 2001

Pagination or Media Count : 22

Abstract : Rab proteins are small CTPases that are essential elements of the protein transport machinery of eukaryotic cells. Each round of membrane transport requires a cycle of Rab protein nucleotide binding and hydrolysis. My research project consists in the study of Rab CTPases,the way in which they regulate intracellular transport, and the elucidation of mechanisms by which proteins involved in intracellular protein trafficking are linked to uncontrolled cellular proliferation and cancer. Our laboratory has recently characterized a protein, Yiplp , which appears to play a role in Rab-mediated membrane transport in Saccharomyes cerevisiae. In a search for a protein accesory factor that may act in conjunction with Yiplp, we discovered the %Familial Adenomatous Polyposis (PAP) locus gene TB2. This gene is adjacent to tumor suppressor genes MCC and APC In this past year I have been able to characterize Yoplp the Saccharomyces cerevisiae homolog of TE2. Yoplp overexpression results in cell death and defects in intracellular trafficking. My project will be to characterize TE2 and its interaction with YIPl and Rab proteins. Our studies will aim to dissect the molecular details of the TB2 gene and its interaction with other proteins through mutagenesis and molecular gene replacement. We will then characterize its role in the breast cancer cell line MCP7 and examine the physiological role of TB2 during mammary epithelial cell differentiation.

Descriptors :   *EPITHELIUM, *CELLS(BIOLOGY), *MAMMARY GLANDS, *FOLIC ACID, *METASTASIS, *BREAST CANCER, MOLECULES, PROTEINS, NEOPLASMS, METABOLISM, MUTATIONS, TRANSPORT, MACHINES, REPLACEMENT, GENES, HYDROLYSIS, DEATH, SUPPRESSORS, NUCLEOTIDES.

Subject Categories : Biochemistry
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE