Accession Number : ADA429514

Title :   Improving Blood Monitoring of Enzymes as Biomarkers of Risk From Anticholinergic Pesticides and Chemical Warfare Agents

Descriptive Note : Annual rept. 24 Sep 2003-23 Sep 2004

Corporate Author : CALIFORNIA UNIV DAVIS

Personal Author(s) : Wilson, Barry W.

PDF Url : ADA429514

Report Date : OCT 2004

Pagination or Media Count : 14

Abstract : Blood biomarkers are an important way to monitor exposure to anticholinergic pesticides and chemical warfare agents and to establish whether some are at greater risk than others from exposure to them. Many clinical and research laboratories use the colorimetric Ellman assay based on the hydrolysis of acetylthiocholine. CHPPM (US Army Center for Health Promotion and Preventive Medicine) uses a slower delta pH method based on that of Michel to monitor 16,000 DOD personnel each year. This year OP exposure was mimicked by treating whole blood from UCD (University of California, Davis) with DFP, to give a range of AChE activity. RBC AChE activity was measured using both the Ellman and delta pH assays at UCD. An estimated conversion factor was calculated. A normal range of AChE activities from the CHPPM delta pH assay was converted to Ellman units. These ranges are important benchmarks for clinical laboratory ChE determinations in the absence of baseline data.

Descriptors :   *ENZYMES, *CHOLINESTERASE INHIBITORS, *CHEMICAL WARFARE AGENTS, *PESTICIDES, *BLOOD CELLS, *ACETYLCHOLINE, HEALTH, MARKERS, PAIN, ARMY, HYDROLYSIS, PROMOTION(ADVANCEMENT), ASSAYING, PH FACTOR, PREVENTIVE MEDICINE, BLOOD VOLUME, CLINICAL LABORATORIES.

Subject Categories : Biochemistry
      Medicine and Medical Research
      Chemical, Biological and Radiological Warfare

Distribution Statement : APPROVED FOR PUBLIC RELEASE