Accession Number : ADA954887

Title :   Composition and Function of Phospholipids in Plasmodium knowlesi.

Descriptive Note : Annual progress rept. no. 2, 1 Oct 73-30 Sep 74,

Corporate Author : MARYLAND UNIV COLLEGE PARK DEPT OF CHEMISTRY

Personal Author(s) : Purdy,W. C.

Report Date : OCT 1974

Pagination or Media Count : 34

Abstract : This work characterizes the phospholipid composition of the parasite P. knowlesi and of the red blood cells and plasma from individual normal and infected rhesus monkeys. Besides continuing investigation of the intact phospholipid composition of various blood fractions to compare with our previous results, we have enlarged our area of concern to attempt to explain the striking differences between normal and infected plasma LPC. We are presently examining blood drawn from animals before and after fasting. Results to date indicate fasting does not cause the decrease in plasma LPC of the infected monkey. We have found a strong correlation between cholesterol-to-cholesterol ester molar ratios and LPC levels in plasma. The ratio is less than one in normal samples where LPC is high and greater than one in infected samples where LPC is low. Using the simplex method for optimization of variables a satisfactory separation of PE, PS, PI, PC, PA, SM and LPC has been achieved on a liquid chromatograph with moving wire detector. An 180 cm Corasil II column is used and a chloroform/methanol/ammonia solvent mixture of 50/35.9/7.0.

Descriptors :   ANIMALS, BLOOD, CHROMATOGRAPHS, DETECTORS, ERYTHROCYTES, FOOD DEPRIVATION, FUNCTIONS, LIQUIDS, MOTION, OPTIMIZATION, PHOSPHOLIPIDS, PLASMODIUM KNOWLESI, RATIOS, RHESUS MONKEYS, SIMPLEX METHOD, VARIABLES, WIRE

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE