Accession Number : ADA955939

Title :   Control of Human Fibroblast Proliferation.

Descriptive Note : Rept. no. 5 (Final),

Corporate Author : CHILDREN'S HOSPITAL WASHINGTON DC BIOCHEMICAL RESEARCH LAB

Personal Author(s) : Houck, John C.

Report Date : 10 JUN 1973

Pagination or Media Count : 43

Abstract : We have found that diploid human fibroblasts, but not heteroploid human fibroblasts, stringently required serum for their multiplication. Using Diaflo ultrafiltration membrane units, isoelectric focusing, and preparative gel electrophoresis, we have isolated and purified this mitogenic activity from mammalian sera. This electrophoretically homogeneous sialoprotein is 120,000 daltons in size, with an isoelectric point of pH 5.2-5.4; it is made up of two electrophoretically identical dimers weighing 57,000 each; it is thermostable and is inactivated by both trypsin and neuraminidase. (jes)

Descriptors :   *DIPLOID CELLS, *FIBROBLASTS, *HUMANS, ELECTROPHORESIS, GELS, GLYCOSIDE HYDROLASES, MEMBRANES, MITOSIS, TRYPSIN, ULTRAFILTRATION.

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE