Accession Number : ADB086796

Title :   Propagation in Serum-Free Medium of Cell Cultures Useful for Vaccine Production.

Descriptive Note : Annual rept. Feb-Aug 81,

Corporate Author : MINNESOTA UNIV AUSTIN HORMEL INST

Personal Author(s) : Jenkin, H. M. ; Malewicz, B. ; Anderson, L. E. ; Crilly, K. S.

Report Date : AUG 1981

Pagination or Media Count : 34

Abstract : Attempts to adapt six diploid cells to grow in monolayer cultures in serum-free media during the past 6 months have met with mixed success. The best results obtained thus far were with rhesus monkey kidney cells (MK-2) which have been passed serially six times in serum-free Waymouth media containing 5 microgram sodium oleate and 2 mg of fatty acid-free bovine serum albumin/m1 (WO5). Human foreskin cells (prepuce-50) have been serially passed two times in serum-free WO20 medium after being grown in WO20 with 2.5% newborn calf serum 11 serial passages. Neither of these two cell types have been serially passed sufficient times to know that they will remain stable. A series of eight media designed in our laboratory, containing different combinations of components found in fetal calf serum or newborn calf serum, were tested to grow African green monkey fetal lung cells (DBS-FRhL-2) in serum-free medium.

Descriptors :   *DENGUE, *VACCINES, *DIPLOID CELLS, PRODUCTION, CULTURE MEDIA, CELLS(BIOLOGY), CULTURES(BIOLOGY).

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE