Accession Number : ADB101194

Title :   Control of Hepatitis Virus Infections by New Methods.

Descriptive Note : Annual progress rept. 1 Jun 82-31 Jan 83,

Corporate Author : BAYLOR COLL OF MEDICINE HOUSTON TX

Personal Author(s) : Melnick, Joseph L.

Report Date : 31 JAN 1983

Pagination or Media Count : 31

Abstract : During the past contract year, we have continued our investigations with an HBsAg synthetic peptide (SP1) which contains amino acid residues 122-137 associated with the ayw subtype sequences of the major envelope polypeptide, P25. Several important immunochemical parameters associated with SP1 were established: 1) Conjugation of SP1 to a tetanus toxoid (TT) carrier or aggregation of SP1 in micelle form yielded material that produced a good booster response in mice. In fact, similar titers were noted in mice injected with 2 doses of alum-precipitated material of either SP1 conjugated to TT or purified HBsAg 22-nm particles. 2) immunization of a chimpanzee with preexisting anti-HBs with 2 inoculations of SP1-TT in alum produced a significant booster antibody response. 3) SP1 contains a conformational a epitope and a sequential y epitope. We have also produced an anti-idiotype reagent in rabbits that recognizes a common idiotypic determinant on human anti-HBs molecules. Finally, we have demonstrated that mice injected with specifically purified antiidiotype produced to human anti-HBs produce immune cells that secrete antibody which reacts specifically with HGsAg. We are currently investigating the potential of this reagent for the specific modulation of the immune response.

Descriptors :   *ANTIBODIES, *IMMUNITY, *HEPATITIS, *ANTIVIRAL AGENTS, ALUMS, CELLS(BIOLOGY), CHEMICAL AGENTS, CHIMPANZEES, CLOSTRIDIUM TETANI, HEPATITIS VIRUSES, IMMUNIZATION, MICE, MODULATION, PEPTIDES, RABBITS, RESPONSE(BIOLOGY), VACCINES, VIRUS DISEASES, ANTIGENS, ANTIGEN ANTIBODY REACTIONS, IMMUNOCHEMISTRY.

Subject Categories : Medicine and Medical Research
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE