Accession Number : ADB101195

Title :   Control of Hepatitis Virus Infections by New Methods.

Descriptive Note : Annual progress rept. 1 Feb 83-31 Jan 84,

Corporate Author : BAYLOR COLL OF MEDICINE HOUSTON TX

Personal Author(s) : Melnick, Joseph L.

Report Date : 31 JAN 1984

Pagination or Media Count : 15

Abstract : During the past contract year, we have continued our investigations with antiidiotype reagents that recognize a common idiotypic determinant on human anti-HBs molecules. Several important immunochemical parameters were established: 1) Injection of anti-idiotype antibodies prior to HBsAg inoculation enhanced the anti-HBs response when compared to groups of mice given control antibodies before HBsAg. 2) Primarily IgG anti-HBs was induced if the anti-idiotype antibodies were given as an alum precipitate, whereas IgM anti-HBs was generated if the anti-idiotype antibodies were administered in a soluble form. 3) The anti-HBs response also was increased when antiidiotype antibodies were given in conjugation with a cyclic synthetic HBsAg peptide (SPI) which contains amino acid sequences 122-137. This anti-HBs response was comparable to a single injection of HBsAg. We also have induced anti-HBs by injecting anti-idiotype antibodies alone. The antiidiotype-induced anti-HBs expressed an interpsecies idiotype that is shared by human anti-HBs produced by natural infection with HBV. In addition, the anti-HBs recognized the group-specific a determinant of HBsAg. These data suggest the potential use of antiidiotypes as vaccines and immunopotentiators of the anti-HBs response. Studies also have been performed on the use of an HBsAg polypeptide vaccine in young adults. Finally, we have made a firm commitment from Baylor funds to continue our investigations with HBsAg synthetic peptides by purchasing an amino acid synthesizer.

Descriptors :   *ANTIBODIES, *ANTIVIRAL AGENTS, *HEPATITIS, *IMMUNITY, ADULTS, ALUMS, AMINO ACIDS, CONTROL, HEPATITIS VIRUSES, HUMANS, INFECTIOUS DISEASES, INJECTION, INOCULATION, MICE, PEPTIDES, SYNTHESIS, VACCINES, VIRUS DISEASES, ANTIGENS, IMMUNOGLOBULINS, IMMUNOCHEMISTRY, ANTIGEN ANTIBODY REACTIONS.

Subject Categories : Medicine and Medical Research
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE