Accession Number : ADB107463

Title :   Cloning of Acetylcholinesterase Gene in a Microbial Vector.

Descriptive Note : Annual Progress rept. May 82-Apr 83,

Corporate Author : CALIFORNIA UNIV SAN FRANCISCO

Personal Author(s) : West, Brian L. ; Baxter, John D.

Report Date : 14 JUL 1983

Pagination or Media Count : 8

Abstract : Efforts made toward the end of cloning synthetic deoxyribonucleic acid (DNA) sequences coding for the enzyme acetylcholinesterase are described. The methods described include the isolation, purification, and analysis of messenger ribonucleic acid (mRNA) from the electric organ of Torpedo californica, a tissue which is highly enriched in acetylchloinesterase. Also described is the construction of three libraries of clones of E. coli bacteria containing the synthetic DNA sequences, and the screening that has been done to isolate a clone or clones containing sequences coding for the enzyme. The conclusion is made that the correct clone(s) have not yet been isolated, although the evidence strongly indicates that at least one bank should contain the clone(s) of interest.

Descriptors :   *DISEASE VECTORS, *ACETYLCHOLINESTERASE, *GENES, *CLONES, *DEOXYRIBONUCLEIC ACIDS, SHARKS, RIBONUCLEIC ACIDS, GENETIC ENGINEERING, SYNTHETIC MATERIALS, ORGANS(ANATOMY), PURIFICATION, LIBRARIES, ESCHERICHIA COLI, ENZYMES, ISOLATION, CODING, SEQUENCES.

Subject Categories : Biochemistry
      Anatomy and Physiology
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE