Accession Number : ADB109905

Title :   Research in Drug Development against Viral Diseases of Military Importance (Biological Testing).

Descriptive Note : Annual rept. 15 Nov 85-15 Nov 86,

Corporate Author : SOUTHERN RESEARCH INST BIRMINGHAM AL

Personal Author(s) : Shannon, W. M. ; Holland, L. E. ; Arnett, G. ; Brando, L. V. ; Hollingshead, M. G.

Report Date : 15 DEC 1986

Pagination or Media Count : 155

Abstract : The purpose of this program is to evaluate the efficacy of candidate antiviral compounds against a spectrum of viruses of military importance; e.g., vaccinia virus (VV) adenovirus (Ad), vesicular stomatitis virus (VSV), Punta Toro virus (PT), sandfly fever virus (SF), Yellow fever virus (YF), Venezuelan equine encephalitis virus (VEE), Japanese encephalitis virus (JE), Pichinde virus (PIC), and Korean hemorrhagic fever virus (KHF), and against human immunodeficiency virus (HIV) and related animal retroviruses including the feline leukemia virus (FeLV) FAIDS variant. The problem invloves a) primary testing of chemical compounds and natural products for antiviral efficacy in vitro using cpe-inhibition or plaque-reduction assays, b) primary testing of materials for antiviral efficacy in two animal model systems in vivo (Venezuelan equine encephalitis virus infections in mice and Pichinde virus infections in hamsters), and (c) secondary evaluation of candidate active antiviral compounds. Of the compounds submitted, over 100 of the compounds were found to exhibit significant antiviral activity against one or more target viruses in vitro. Potent and selective new antiviral agents were found with significant activity against VV, PT, SF, AD, PIC, and VSV. Several compounds were found to be active against FeLV (FAIDS), but these were not as effective as the positive control drug 2',3'-dideoxycytidine (ddC). The antiviral efficacy of Ribavirin in the treatment of lethal Pichinde virus infections in MHA strain hamsters was confirmed and the animal model was developed for antiviral screening in vivo.

Descriptors :   *ANTIVIRAL AGENTS, ADENOVIRUSES, IN VITRO ANALYSIS, BIOLOGY, TEST METHODS, DRUGS, ANIMALS, MODELS, EFFICIENCY, IN VIVO ANALYSIS, CHEMICAL COMPOUNDS, HAMSTERS, HEMORRHAGIC FEVERS, KOREA, VIRUSES, SECONDARY, STRAINS(BIOLOGY), VESICULAR STOMATITIS, VIRUS DISEASES, JAPANESE ENCEPHALITIS VIRUSES, MICE, SANDFLY FEVER VIRUS INFECTION, SPECTRA, VACCINIA VIRUS, VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS, YELLOW FEVER VIRUS.

Subject Categories : Medicine and Medical Research
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE