Accession Number : ADB134456

Title :   Evaluation of Membrane Systems for Washing/Deglycerolizing Packed Red Blood Cells.

Descriptive Note : Final rept. 10 Feb 86-9 Feb 88,

Corporate Author : BEND RESEARCH INC OR

Personal Author(s) : Pearson, K. R. ; Radovich, J. M. ; Schofield, R. W. ; Wedel, R. L.

Report Date : 05 JAN 1989

Pagination or Media Count : 76

Abstract : The objective was to develop a membrane-based process for deglycerolizing previously frozen, packed red blood cells (RBCs) prior to transfusion. The membrane-based process must reduce the glycerol concentration in a unit of RBCs to less than 1 wt% using about 2000 ml of wash solution in 35 to 45 min. The plasma hemoglobin (Hb) concentration in the washed RBCs must be less than 150 mg/dl. We met the objectives of the program. We successfully demonstrated the feasibility of using commercially available membrane devices (hemodialyzers, hemofilters, and plasmapheresis devices) to deglycerolize packed RBCs. Our optimization studies showed that the deglycerolization performance be improved by concentrating the RBCs before washing and by promoting better mixing of the RBCs and the wash solution within the blood bag. (kt)

Descriptors :   *MEMBRANES, *BIOCHEMISTRY, BAGS, BLOOD, BLOOD PLASMA, BLOOD TRANSFUSION, HEMOGLOBIN, OPTIMIZATION.

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE