Accession Number : ADB137465

Title :   Primary Structure of Nicotinic Acetylcholine Receptor.

Descriptive Note : Annual rept. 15 Jul 87-31 Dec 88; Final rept. 15 Jul 85-31 Dec 88,

Corporate Author : SALK INST FOR BIOLOGICAL STUDIES LA JOLLA CA

Personal Author(s) : Patrick, James W.

Report Date : JAN 1989

Pagination or Media Count : 46

Abstract : Chemical signalling between cells is an important form of biological regulation. We have focussed our attention on the signalling mechanisms that use acetylcholine, cholinergic receptors and acetylcholinesterase. Our approach to understanding these mechanisms has been to clone DNA sequences that encode the proteins that comprise the acetylcholine receptor molecules and to determine the primary structure of the receptors from these recombinant DNA clones. In the course of this work, we have discovered that there is family of genes that encode a family of receptor molecules. Through our research we have determined the primary structure of these proteins, shown that they can be expressed in Xenopus oocytes to form functional receptors, shown that the receptors have different properties, and shown that the receptors are expressed in different parts of the brain. This unexpected prevalence and diversity of nicotinic acetylcholine receptors changes the way we think about the drugs and toxins that affect cholinergic signalling. Because these receptors are pharmacologically distinguishable and occur in regions of the brain with different known functions one must consider the idea that cholinergic ligands exert differential effects in different brain pathways. Keywords: Nerve transmission; Neurochemistry; RA 5; Neuromuscular junction; Synapse; Acetylcholine receptor; Recombinant DNA; Oocyte expression; DNA sequencing; Pharmacology. (KT)

Descriptors :   *ACETYLCHOLINE, *CHEMORECEPTORS, *MOLECULAR STRUCTURE, *NICOTINE, ACETYLCHOLINESTERASE, BRAIN, CHEMICALS, CHOLINERGIC NERVES, CLONES, DEOXYRIBONUCLEIC ACIDS, DRUGS, GENETIC ENGINEERING, JUNCTIONS, LIGANDS, MOLECULES, MUSCLES, NERVE TRANSMISSION, NERVES, NEUROCHEMISTRY, PHARMACOLOGY, PROTEINS, REGIONS, RESPONSE(BIOLOGY), SENSE ORGANS, SEQUENCES, SIGNALS, SYNAPSE, TOXINS AND ANTITOXINS.

Subject Categories : Biochemistry
      Biology
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE