Accession Number : ADB145691

Title :   Chemical Blistering: Cellular and Macromolecular Components.

Descriptive Note : Annual rept. 1 Jan-31 Dec 87,

Corporate Author : MICHIGAN UNIV ANN ARBOR

Personal Author(s) : Bernstein, I. A.

Report Date : 31 JAN 1988

Pagination or Media Count : 56

Abstract : The overall purpose of this investigation is to elucidate the molecular mechanisms by which bis(beta-chloroethyl) sulfide (BCES) exerts its vesicant action when applied topically to human skin by establishing morphological, cytochemical and/or biochemical indicators of mustard toxicity in cultures of keratinocytes and examining and evaluating the relevance of these parameters to vesication. During the second year of this project, the investigation has attempted to (a) confirm that DNA is the macromolecular system which is most sensitive to damage in cultures exposed to BCES, (b) identify the lowest level of exposure at which the lesion in DNA can be observed and the level of exposure at which the lesion in DNA can be observed and the level of exposure at which the lesion cannot be repaired. Keywords: Mustard, Keratinocyte, Tissue culture, ALkylation, Toxicity, Chemical blistering, Mitochondria, Metabolism, DNA Repair, Epidermal proliferation, Epidermal differentiation. (JES)

Descriptors :   *CHEMICAL INDICATORS, *DEOXYRIBONUCLEIC ACIDS, *MUSTARD AGENTS, ALKYLATION, BIOCHEMISTRY, CELLS(BIOLOGY), DAMAGE, EXPOSURE(GENERAL), HUMANS, MACROMOLECULES, METABOLISM, MITOCHONDRIA, MOLECULAR PROPERTIES, REPAIR, SENSITIVITY, SKIN(ANATOMY), TISSUE CULTURE, TOXICITY, VESICANTS.

Subject Categories : Chemical, Biological and Radiological Warfare

Distribution Statement : APPROVED FOR PUBLIC RELEASE