Accession Number : ADB160703

Title :   Development of a Short-Term In-Vitro Dermal Toxicity Screening Test Utilizing Human Cells. Phase 1.

Descriptive Note : Final rept.,

Corporate Author : NORTH AMERICAN SCIENCE ASSOCIATES INC NORTHWOOD OH

Personal Author(s) : Hume, R. D.

Report Date : 15 FEB 1990

Pagination or Media Count : 16

Abstract : There is a large effort underway in the world today to identify and develop in-vitro alternative tests to supplement or replace current in vivo toxicity test methods. The Agarose Diffusion Method is an excellent candidate for development as a short term in-vitro dermal toxicity screening test using human cells. The Phase I effort has adapted the current Agarose Diffusion Method for use in screening for primary skin irritation. This required manipulation of the agarose thickness and the investigation of an alternative cell type. The human foreskin cell line has been adapted to the test method. Of particular interest is the similarity of the human cell line with the L-929 mouse fibroblast cell line. The test system developed employed an agarose overlay medium volume of 10 milliliters, with final concentrations of 1% agarose, 5% fetal bovine serum supplemented Minimum Essential Media (Eagle), and 0.01% neutral red. Prepared samples were applied to the agarose surface in a 25 cm2 sealed flask via filter paper discs.

Descriptors :   , CELLS, CELLS(BIOLOGY), DISKS, FILTER PAPER, HUMANS, IRRITATION, SKIN(ANATOMY), TEST AND EVALUATION, TEST METHODS.

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE