Accession Number : ADB170987

Title :   A Gene Probe Amplification System Optimized for Direct Imaging Detection.

Descriptive Note : Final rept.,

Corporate Author : EASTERN ANALYTICAL INC COLLEGE PARK MD

Personal Author(s) : Moore, Larry J.

Report Date : 15 SEP 1992

Pagination or Media Count : 35

Abstract : The key feasibility elements have been completed that are expected to lead to a portable field system for rapid water analysis using gene probe amplification techniques. A filtration system has been devised to separate E. coli bacteria from other species and debris in lake water samples. Cell lysing techniques for E. coli have been investigated and compared for efficiency. Gene amplification techniques also have been investigated and optimized for detection of E. coli DNA. Separation and detection of the amplification products were conducted using gel electrophoresis and silver staining. Lysing methods developed have reduced the time required for this step severalfold and may be reduced to a negligible level in Phase II research. Based on this Phase I feasibility investigation, we estimate that a field portable system may be developed for water quality analysis that requires fewer than 2 to 3 hours.... Water, Pathogen detection, Gene probe, E. Coli, SBIR, RA II.

Descriptors :   *GENES, *ESCHERICHIA COLI, *CULTURES(BIOLOGY), BACTERIA, CELLS(BIOLOGY), DEBRIS, DETECTION, EFFICIENCY, ELECTROPHORESIS, ESTIMATES, FILTRATION, GELS, LAKES, PHASE, PROBES, SEPARATION, WATER ANALYSIS, WATER QUALITY, SILVER COMPOUNDS, DEOXYRIBONUCLEIC ACIDS, STRAINS(BIOLOGY), MEMBRANES(BIOLOGY), VIRUSES, ENZYMES, MICROORGANISMS.

Subject Categories : Medicine and Medical Research
      Medical Facilities, Equipment and Supplies

Distribution Statement : APPROVED FOR PUBLIC RELEASE