Accession Number : ADD003619

Title :   Method of Isolating and Cryopreserving Human White Cells from Whole Blood.

Descriptive Note : Patent,

Corporate Author : DEPARTMENT OF THE NAVY WASHINGTON D C

Personal Author(s) : Lionetti,Fabian J ; Allston,M Hunt

Report Date : 25 Jan 1977

Pagination or Media Count : 12

Abstract : A method of salvaging granulocytes, or white cells, from buffy coat of centrifuged whole blood is provided. The sequence of operations leading to the separation red cells, white cells, platelets and plasma is presented in a flow diagram of a bag system for the cryopreservation of leukocytes, with freezing of the white cells accomplished by the introduction of a combination of hydroxyethyl starch (HES), which functions as both a sedimenting agent and a cryoprotective agent, and dimethylsulfoxide (DMSO), a cryoprotective agent. A preferred combination is 4% HES with 5% DMSO. White cell separation is interfaced with collection methods for plasma and platelets so as to conserve all major cell types. (Author)

Descriptors :   *Patents, *Blood preservation, *Leukocytes, Blood banks, Freezing, Cryoprotective agents

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE