Accession Number : ADD008885

Title :   Method for Producing a Vaccine Against Bacterial Infections Caused by Pseudomonas Aeruginosa.

Descriptive Note : Patent,

Corporate Author : DEPARTMENT OF THE ARMY WASHINGTON DC

Personal Author(s) : Pier,Gerald B ; Sadoff,Jerald C

Report Date : 25 Aug 1981

Pagination or Media Count : 7

Abstract : A method for isolating a non toxic, high molecular weight polysaccharide antigen from the crude slime of a Pseudomonas aeruginosa culture, and a method for inducing immunity in a host to said live organisms is described. The slime is initially prepared for the isolation procedure by separating the bacterial cells from the slime and dissolving the slime in a phosphate buffer solution. Dissolved contaminating nucleic acids are then precipitated and separated from solution. A lipid A portion of the contaminating lipopolysaccharide constituent is then removed and precipitated by acetic acid hydrolysis, and the remaining lipids present are extracted with chloroform. Nearly all of the residual nucleic acids are then removed by digestion with nucleases, and the remaining protein extracted with phenol. The aqueous and phenol layers are then separated, and the aqueous layer applied to a gel filter to isolate the antigen by column chromatography. The antigen appears in the void volume. A vaccine containing the polysaccharide antigen of this invention has been successfully tested and found to be non toxic and highly effective in inducing an immune response to the organism in a host. (Author)

Descriptors :   *Patents, *Vaccines, Bacterial diseases, Antigens, Polysaccharides, Isolation, Pseudomonas aeruginosa, Production, Infectious diseases, Immunity, Lipopolysaccharides, Lipids

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE