Accession Number : ADD012163

Title :   Magnetic Separation Device.

Descriptive Note : Patent Application,

Corporate Author : DEPARTMENT OF THE NAVY WASHINGTON DC

Personal Author(s) : Starken,George M ; Cavin,J ; Quinnell,Richard A ; Twigg,Charles A ; Crawford,Duncan P

Report Date : 25 Sep 1985

Pagination or Media Count : 28

Abstract : This invention pertains to magnetic separation devices and more particularly to; magnetic separation devices used to selectively remove magnetic bead-coated cells from tissues such as bone marrow or blood. Depletion of cell populations from boner marrow has been mainly approached using antibodies conjugated to toxins such as ricin or with antibodies complement to effect lysis of the target cells. These techniques have severals disadvantages including difficulty in measuring the selective cell kill in the marrow, non-specific toxicity of either toxins or complement, and the necessity to prepare large amounts of complement. In addition, many antibodies are neither cytotoxic with complement nor toxin conjugates. Polymeric microspheres conjugated to antibodies have been used to probe the cell surface for receptor sites using scanning electron microscopy. Iron-containing polymeric microspheres tagged with fluroescent dyes conjugated to antibodies were used to separate red blood cells and lymphoid cells by binding the antibody-microsphere to selected cells and exposing the cell population to a magnetic field. Over 99% of the bound cells were attracted by the magnet.

Descriptors :   *PATENT APPLICATIONS, *MEDICAL EQUIPMENT, *LYMPHOCYTES, *MAGNETIC DEVICES, *MAGNETIC SEPARATION, *ERYTHROCYTES, ANTIBODIES, BLOOD, BONE MARROW, CELLS(BIOLOGY), DEPLETION, ELECTRON MICROSCOPY, INVENTIONS, IRON, LYSIS, MAGNETIC FIELDS, MICROSPHERES, POLYMERS, RICIN, SENSE ORGANS, SEPARATORS, SITES, SURFACES, TARGETS, TOXICITY, TOXINS AND ANTITOXINS, BLOOD CELLS, IMMUNOLOGY, FLUORESCENT DYES

Subject Categories : Medicine and Medical Research
      Medical Facilities, Equipment and Supplies

Distribution Statement : APPROVED FOR PUBLIC RELEASE