Accession Number : ADD012173

Title :   Quantitative Immunochromatographic Strip Assay Method and Apparatus.

Descriptive Note : Patent Application,

Corporate Author : DEPARTMENT OF THE ARMY WASHINGTON DC

Personal Author(s) : Hamont,John E van ; Vincent,Jack W ; Pappas,Michael G ; Hajkowski,Ruta

Report Date : 18 Oct 1985

Pagination or Media Count : 22

Abstract : This patent application discloses an immunochromatographic assay for quantitation of a biological substrate such as hepatitis B antibody in which strips of nitrocellulose acetate filter paper are uniformly coated with a specific binding material for the substrate, such as hepatitis B surface antigen, after which non-specific binding sites on the strips are blocked with proteinaceous material, such as serum albumin. Sample solutions containing unknown amounts of substrate are allowed to migrate up each strip so that the specific binding material will bind the substrate, the area occupied by bound substrate corresponding to the concentration of substrate in the sample solution. The presence of bound substrate is determined by immersing in a solution containing a complex of specific antibody for the bound substrate, such as anti-immunoglobulin, and an indicator activating agent, such as peroxidase enzyme. The strips are then rinsed to remove excess unbound complex, and immersed in an indicator solution, such as a solution of 4-chloro-1-naphthol and hydrogen peroxide. Peroxidase mediated oxidation of the indicator produces a dark blue chromophore precipitate marking the presence of substrate on the strip.

Descriptors :   *PATENT APPLICATIONS, *IMMUNOASSAY, *CHROMATOGRAPHIC ANALYSIS, *ANTIGEN ANTIBODY REACTIONS, ENZYMES, FILTER PAPER, HYDROGEN PEROXIDE, INDICATORS, ACETATES, ACTIVATION, NITROCELLULOSE, OXIDATION, ANTIBODIES, BINDERS, PEROXIDASES, SERUM ALBUMIN, SOLUTIONS(GENERAL), SUBSTRATES, BANDS(STRIPS), TEST EQUIPMENT, HEPATITIS VIRUSES

Subject Categories : Medicine and Medical Research
      Physical Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE