Accession Number : ADD012346

Title :   Flow Cytometric Measurement of Total DNA and Incorporated Halodeoxyuridine.

Descriptive Note : Patent,

Corporate Author : DEPARTMENT OF THE AIR FORCE WASHINGTON DC

Personal Author(s) : Dolbeare,Frank A ; Gray,Joe W

Report Date : 29 Apr 1986

Pagination or Media Count : 16

Abstract : A method for the simultaneous flow cytometric measurement of the total DNA content and the level of DNA synthesis in normal and malignant cells is disclosed. The sensitivity of the method allows a study of cell cycle traverse rates for large scale cell populations as well as single cell measurements. A DNA stain such a propidium iodide is used as the probe for the measurement of total DNA content and a monoclonal antibody reactive with a DNA precursor such as bromodeoxyuridine (BrdU) is used as a probe for the measurement of BrdU uptake by the cells as a measure of DNA synthesis.

Descriptors :   *PATENTS, *CYTOCHEMISTRY, *DEOXYRIBONUCLEIC ACIDS, *URIDINE, *BIOSYNTHESIS, CELLS(BIOLOGY), NEOPLASMS, MEASUREMENT, HISTOCHEMISTRY, BIOLOGICAL STAINS, ANTIBODIES, CANCER, CHEMOTHERAPY, MEDICAL RESEARCH

Subject Categories : Biochemistry
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE