Accession Number : ADD012400

Title :   Method for Separating and Measuring the Amount of Polar Compounds and Their Metabolites in Aqueous Solutions.

Descriptive Note : Patent,

Corporate Author : DEPARTMENT OF THE ARMY WASHINGTON DC

Personal Author(s) : Ellin,Robert I ; Zvirblis,Peter

Report Date : 20 May 1986

Pagination or Media Count : 8

Abstract : Polar compounds such as pyridostigmine are used extensively in the treatment of patients with myasthenia gravis. Recently, pyridostigmine, in combined therapy with atropine and an oxime, was suggested as an antidote in organo phosphate poisonings. In cases where patients received oral doses of pyridostigmine, considerable inter-subject variation of blood levels was reported. Maximum plasma levels after similar oral doses administered to man range from 1 to 200 mg/ml. The quantitative isolation and accurate determination of the drug in biological fluids would result in establishing pharmacokinetic constants for pyridostigmine administered alone and in combination with other drugs. This invention relates to a novel method for separating and measuring the amount of polar compounds, such as pyridostigmine, acetylcholine, neostigmine, and edrophonium, and their metabolites in aqueous solutions. The method is used to determine the urinary excretion and plasma levels of polar compounds administered to animals.

Descriptors :   *PATENTS, *DRUGS, *METABOLITES, *PHARMACOKINETICS, *QUANTITATIVE ANALYSIS, ACETYLCHOLINE, ANIMALS, ANTIDOTES, ATROPINE, BLOOD PLASMA, BLOOD VOLUME, CHEMICAL COMPOUNDS, CONSTANTS, DETERMINATION, EXCRETION, HUMANS, ISOLATION, LEVEL(QUANTITY), MEDICAL SERVICES, ORAL DOSAGE, OXIMES, PARASYMPATHOMIMETIC AGENTS, POLARITY, SOLUTIONS(MIXTURES), THERAPY, URINE, WATER, BIOASSAY, BODY FLUIDS, BLOOD CHEMISTRY, CHEMOTHERAPY, SEPARATION, LIQUID CHROMATOGRAPHY

Subject Categories : Biochemistry
      Pharmacology
      Physical Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE