Accession Number : ADD012549
Title : Hybridoma Cell Lines amd Monoclonal Antibodies to Clostridium difficile Toxins A and B.
Descriptive Note : Patent Application,
Corporate Author : DEPARTMENT OF THE ARMY WASHINGTON DC
Personal Author(s) : Rothman,Sara ; Gentry,Mary K
Report Date : 30 May 1986
Pagination or Media Count : 26
Abstract : Clostridium difficile, the etiological agent of pseudomembranous colitis in humans and animals, produces two toxins, designated toxin A and toxin B, that are cytotoxic for tissue-cultured mammalian cells. Toxin B is approximately 1,000-fold more cytotoxic than toxin A per mg of protein. Toxin A also possesses enterotoxin activity and causes a fluid response when injected into ligated rabbit intestinal loops. These antibodies produced by a family of murine hybrid cell lines are cross-reactive with both toxin A and toxin B of Clostridium difficile. The monoclonal antibodies are useful in an immunoassay for toxin A and toxin B of C. difficile.
Descriptors : *PATENT APPLICATIONS, *MYELOMA, *ANTIBODIES, *BACTERIAL TOXINS, *CLOSTRIDIUM, *IMMUNOASSAY, CYTOTOXIN, ETIOLOGY, COLITIS, TISSUE CULTURE CELLS, MICE, ENTEROTOXINS, RABBITS, INTESTINES, RADIOIMMUNOASSAY, INVENTIONS
Subject Categories : Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE