Accession Number : ADD012743

Title :   Sensitive Radioimmunoassay Using Antibody to L-Hyoscyamine.

Descriptive Note : Patent,

Corporate Author : DEPARTMENT OF THE ARMY WASHINGTON DC

Personal Author(s) : Verma,Pritam S

Report Date : 27 May 1986

Pagination or Media Count : 13

Abstract : A sensitive and specific radioimmunoassay is developed for L-hyoscyamine resulting from atrophine administration with which a concentration as low as 25 pg/ml using 0.1 ml of sample can be measured without the need for extraction. Specificity studies indicate that an antibody according to this invention has high specific recognition of L-hyoscyamine with only about 37% cross reaction with the D-hyoscyamine enantiomer of atropine. The antibody is produced from an immunogen having conjugated at least 42 and preferably 45 L-hyoscyamine-p-aminobenzoic acid haptenic molecules per molecule of bovine serum albumin. An antibody according to this invention can be used with a dilution titer as high as 1:2000.

Descriptors :   *PATENTS, *RADIOIMMUNOASSAY, *ANTIBODIES, *BELLADONNA ALKALOIDS, *ANTIDOTES, PHARMACOKINETICS, SERUM ALBUMIN, BOVINES, IMMUNOGENS, MOLECULES, RECOGNITION, SENSITIVITY, ATROPINE

Subject Categories : Biochemistry
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE