Accession Number : ADD013676

Title :   Method of Producing a Plaque Dispersing Enzyme.

Descriptive Note : Filed 9 Feb 81, patented 4 May 82,

Corporate Author : DEPARTMENT OF THE NAVY WASHINGTON DC

Personal Author(s) : Simonson, Lloyd G ; Lamberts, Burton L

Report Date : 04 May 1982

Pagination or Media Count : 4

Abstract : The present invention relates to an enzyme useful as an oral therapeutic agent and more particularly to a method of producing alpha-1, 3-glucan 3-glucanohydrolase which can be used to attack glucans of dental plaque. Dental plaque is a deposit which accumulates on the teeth and adjacent surfaces in the oral cavity. The plaque is a product of microbial growth resulting from food residues in the mouth. Mucoproteins and minerals present from the saliva and dead cells in the mouth also assist in plaque formation. A method of producing alpha-1, 3-glucanase by introducing a bacterial culture such as Pseudomonas sp. isolate NRRL B-12324 into an aqueous medium containing a 'limit glucan' substrate which is greater than 90 percent alpha-1, 3-glycosidically linked, then allowing growth to take place to accumulate a-1, 3-glucanase, and then recovering the enzyme for use as an oral therapeutic agent.

Descriptors :   *PREVENTIVE DENTISTRY, *GLUCANASES, CHEMOTHERAPEUTIC AGENTS, DENTAL PLAQUE, ENZYMES, FOOD, GLYCOPROTEINS, GROWTH(PHYSIOLOGY), MICROORGANISMS, MINERALS, MOUTH, MUTAGENS, PSEUDOMONAS, RESIDUES, SALIVA, SURFACES, TEETH, WATER, CELLS(BIOLOGY), BIODETERIORATION, CULTURES(BIOLOGY), PATENTS

Subject Categories : Pharmacology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE