Accession Number : ADD013890

Title :   Scaled-Up Production of Liposome-Encapsulated Hemoglobin.

Descriptive Note : Patent, Filed 29 Aug 86, patented 11 Oct 88,

Corporate Author : DEPARTMENT OF THE NAVY WASHINGTON DC

Personal Author(s) : Farmer, Martha C ; Beissinger, Richard L

Report Date : 11 Oct 1988

Pagination or Media Count : 12

Abstract : Objects of the present invention are to provide: 1. A blood substitute or blood extender, for administration to human or animal patients; 2. A substitute which is also an oxygen carrier, based upon hemoglobin, capable of circulating many hours within the circulatory system of the recipient and having adequate oxygenation characteristics, and consisting of hemoglobin-containing liposomal vesicles which are of nearly a uniform size, averaging 0.2 microns in diameter, having generally a simple lipid bilayer and which are sterilizable; 3. A process for preparing a blood substitute or blood extender without chemical reaction to modify native hemoglobin, and via an aseptic process capable of yielding at least 10 ml/min of sterile product; 4. A blood substitute or blood extender which has a half-life, as measured in mice, of at least 15 hours; and 5. A method or producing blood substitutes or blood extenders that is a large-batch or continuous flow encapsulation system which can be easily scaled up. A method for making sterile liposome-encapsulated hemoglobin having an oxygen carrying capacity of 20 vol/%, a half life of 15-20 hours as measured in mice, with the liposome made from chloroform, HSPC, cholesterol, negatively charged DMPG and alpha-tocopherol is disclosed. Keywords: Patents. (aw)

Descriptors :   *BLOOD SUBSTITUTES, *HEMOGLOBIN, *PATENTS, ANIMALS, ASEPSIS, BLOOD, CARDIOVASCULAR SYSTEM, CHEMICAL REACTIONS, CHLOROFORM, CHOLESTEROL, CYTOPLASM, ENCAPSULATION, FLOW, HALF LIFE, HUMANS, LIPIDS, MICE, OXYGEN, PATIENTS, PRODUCTION, SCALING FACTORS, SIZES(DIMENSIONS)

Subject Categories : Biochemistry
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE